Biotinylated hyaluronan binding protein
WebR.A. Kohanski, in Encyclopedia of Biological Chemistry (Second Edition), 2013 Abstract. The biotinylation of proteins is the covalent coupling of biotin to an amino acid or … WebJan 21, 2024 · Hyaluronan-binding protein (HABP) from bovine nasal cartilage (Merck Cat # 385910). Biotinylated-HABP from bovine nasal cartilage (Merck Cat # 385911). ...
Biotinylated hyaluronan binding protein
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WebHyaluronan-binding protein in ductal branching morphogenesis, and absence is associated endothelial cell morphogenesis. J. Cell Biol. 119, 643-652. with substantial alterations in the branching. ... Matrix 12, 397-403. Identification of hyaluronan binding proteins using a biotinylated hyaluronan Sugimura, Y., Cunha, G. and Bigsby, R. … WebThe widespread occurrence of hyaluronan-binding proteins indicates that the recognition of hyaluronan is important to tissue organisation and the control of cellular behaviour. ... have also been identified. In the present study biotinylated hyaluronan (bHA) was prepared by reacting adipic dihydrazide with a 170 kDa hyaluronan sample using the ...
Webby the hyaluronan-binding protein. No correlation was found between the degree of HA deposition in the dermis and the expression of CD44 in the skin of shar-pei dogs affected or unaffected by cutaneous mucinosis. A clear positive correlation was found between the existence of clinical mucinosis and the serum HA concentration. In control dogs, serum WebA convenient and reliable method for preparing and using biotinylated hyaluronan for detection of hyaluronan-binding proteins is described. The biotinylated hyaluronan …
WebIn this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1,2). The b-PG is derived from cartilage and consists of a … WebApr 12, 2024 · Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and proteinbinding sequences that contain two αhelices and two antiparallel βsheets (1, 3). There are three categories of link modulecontaining proteins.
WebSep 1, 2007 · A hyaluronan binding protein (HABP) affinity column was used to purify the hyaluronan-containing complex from hypertrophic adipocytes. Briefly, streptavidin-agarose (Sigma) was mixed with biotinylated-HABP (Seikagaku) in PBS containing 50% glycerol and shaken overnight at 4°C. Hyaluronan was added in PBS to protect the HABP from …
WebIn this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan ( 1, 2 ). The b-PG is derived from cartilage and consists of a trypsin fragment of the proteoglycan core protein and one of the link proteins. northern virginia imax theaterWebJan 21, 2024 · Biotinylated hyaluronan-binding protein; biotinylation of hyaluronan-binding protein was performed with biotin-aminocaproic acid sulfo-NHS (Uppsala, Sweden). Hermes-3 anti-CD44 antibody (kind gift from Professor Sirpa Jalkanen, University of Turku, Finland [ 10 , 11 ]). northern virginia hokie barsWebFigure Legend Snippet: Hyaluronan-rich septa are largely unchanged throughout mammary gland development. Immunofluorescence microscopy for hyaluronic acid binding … northern virginia housing marketWebHyaluronan Binding Protein [HABP] (Biotin Conj.) Hyaluronan Binding Protein [HABP] (Biotin Conj.) Product Code. AMS.HKD-BC41. Skip to the end of the images gallery . Skip to the beginning of the images gallery . $640.00. Qty. Add to Cart. Request Quote for Bulk … northern virginia imaging llcWebJul 30, 2024 · Hyaluronan Binding Protein Assay. To localize hyaluronan (HA) in histologic sections, we performed a fluorescent HA binding protein assay ... After a rinse in PBS, biotinylated-HABP (1:100 in normal goat serum, Millipore-Sigma) was added to the slides for 1 h at room temperature. Next, the slides were washed with PBS 4 times and … how to save a contact group in outlook 365WebGene expression by qRT-PCR was analyzed utilizing a panel of HA-related genes: hyaluronan synthesis ( Has1-3 ), hyaluronidases ( Hyal1,2 ), and hyaluronan receptors ( Cd44, Rhamm, Lyve1 ). Third and fourth mammary glands were pooled from 3–4 mice per sample (n). Error bars represent standard error of the mean. how to save a corrupted fileWebOct 1, 2002 · Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe. Connect Tissue Res. 1993. 30: 117-126. Google Scholar. ... Regulation of cellular phosphorylation of hyaluronan binding protein and its role in the formation of second messenger. Biochim Biophys Acta. 1997. 1336: 387-393. Google Scholar. how to save a command line script