WebThis equipment from CTL is specially designed for reading ELISpot plates and FluoroSpot plates. The reader is an image-acquiring and analysis system. It is equipped with a microscope that can scan 96-well plates mounted on a workstation. The accompanied software enables the recognition and quantitation of immunospots in each well. WebApr 14, 2024 · The production of IFN-γ was determined using pre-coated ELISpot plates and a standardized detection system (T-Track ® ELISpot kit, Mikrogen GmbH, Neuried, Germany). PBMCs were incubated in 150 µL AIMV medium (Gibco, Grand Island, NE, USA) at 37 °C for 1 day.
FluoroSpot Kits - Mabtech
WebOct 10, 2024 · Add cells and stimuli to the plate Most cell sources can be used – as long as the cells are alive. FluoroSpot can be applied to basically any cells to... Choose a cell culture medium appropriate for your cells. … 1. Antibody coating: Throughout the ELISpot Assay technique, different substances are added to and washed away from wells. Wells are found on a laboratory plate with tiny dishes/bowls that can be filled with a substance to be examined; the amount of wells on a plate varies, but it usually ranges from 16-100. The first substance added to the wells are cytokine specific monoclonal antibodies. These antibodies coat the walls of the wells for future binding to cytokine. The mono… simply sparkle llc
The ELISpot Assay Enables Functional Analysis of …
Web酶联免疫斑点实验 (enzyme linked immunospot assay, ELISpot) 是一种在96孔板内的一百万个细胞中实现对单个分泌细胞进行定量检测的新型的免疫酶技术。 灵敏度高,易操作,成本相对流式细胞术较低,已被广泛应用于癌症研究,疾病探索以及疫苗研发等领域。 ELISpot原理: 将细胞置于包被有特异性抗体的96孔板中培养,在有刺激的条件下,细胞分泌的细 … http://www.laizee.com/zh_cn/news/view/ELISpot-and-FluoroSpot.html WebAID iSpot EliSpot FluoroSpot Reader - analyze enzymatic and fluorescence EliSpot plates 3-colour FluoroSpot analysis The basic EliSpot/FluoroSpot Reader The AID i Spot is the most successful … simply sparkle