Graphene based gene transfection

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August undefined, 2024

WebJul 1, 2024 · Fig. 1. Illustration of various carbon-based structures (graphite sheets, graphene, nanotubes, fullerene, and nanodiamond) that are frequently used in medicine and biomedical applications. 2. Gene therapy and carbon structures. Gene therapy simply means using therapeutic nucleic acids as drugs to treat diseases [7]. WebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical …

Graphene-based nanomaterials for drug delivery and

WebDec 1, 2024 · Graphene and its derivatives, as sheet-like carbonic nanomaterials, have been progressively utilized in various biomedical fields. Currently, applications of … WebApr 1, 2024 · Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in co … port orchard homeless

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WebJan 23, 2024 · Recently graphene has been used in biomedical applications including DNA sequencing 10, biosensor development 11, and graphene-enhanced cell differentiation … WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene … WebThe use of NA is gaining increasing interest and more than 2000 clinical trials of NA-based therapies are underway. 2 The current advancements in medicine, biology and biotechnology have extended the scope of gene therapy from the “simple” transfection of plasmid DNA (pDNA) to the delivery of a variety of NA, including short interfering RNA ... port orchard hospital

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Graphene-Based MicroRNA Transfection Blocks Preosteoclast Fusion …

WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 … port orchard hourly weatherWebApr 20, 2024 · Graphene-based nanomaterials have been used as scaffolds for cell culture and tissue engineering and have exhibited minimal harmful effect on the mammalian cells, very good adhesion properties, excellent gene transfection efficacy, high capability of the promotion of differentiation of stem cells into bone cells in a controlled manner for bone ... iron man toys infinity war

"WebObjectives: This review describes the different dimensions of carbon-based nanocarriers including 0-D fullerene, 1-D Carbon Nanotubes (CNTs), and 2-D graphene and Graphene Oxide (GO) and their surface modification with different biocompatible and biodegradable molecules via covalent or non-covalent functionalization. " - Graphene based gene transfection

Graphene based gene transfection

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast …

WebFeng, L., Zhang, S., & Liu, Z. (2011). Graphene based gene transfection. Nanoscale, 3(3), 1252. doi:10.1039/c0nr00680g WebJan 6, 2024 · Graphene oxide for transfection. ThermoFisher Scientific describes transfection as the process of artificially introducing nucleic acids – DNA or RNA – into cells, utilising means other than viral infection. A paper published in Nanoscale 2011 titled ‘Graphene based gene transfection’ showed that graphene oxide (“GO”) bound with ...

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WebJun 10, 2013 · Graphene oxide (GO) has been extensively explored in nanomedicine for its excellent physiochemical, electrical, and optical properties. ... 1 Jiangsu Key Laboratory for Carbon-Based, Functional Materials & Devices, ... remarkably enhanced plasmid DNA transfection efficiencies induced by the NIR laser are achieved using NGO-PEG-PEI as … WebJun 18, 2024 · Graphene-oxide-based nanocarriers have shown great potential in gene transfection and could be used in breast cancer treatments by overcoming the limitations and side effects of chemotherapy. This carbon-based material allows a facile surface modification to enhance its biological application and gene delivery efficiency.

WebGene therapy has been found to hold tremendous potential for the treatment of cancers beyond conventional methods. 30–32 Nonviral vector provides safer methods without the potential side effects of viral transfection, such as immunogenicity and carcinogenesis. 33 GO has been considered an ideal vector for gene delivery. 34,35 In this study ... WebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer …

WebJan 27, 2011 · Our results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non … WebDec 4, 2024 · In treating osteoporotic ovariectomized mice, GO‐PEI‐miR‐7b significantly enhances bone mineral density, bone volume as well as bone vascularization through increasing CD31hiEmcnhi cell number. The objective of this study is to design a graphene‐based miRNA transfection drug delivery system for antiresorptive therapy. …

WebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ...

WebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available. iron man toyWebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … port orchard hotel washingtonWebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … iron man toys on amazonWebSep 15, 2012 · Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. … iron man toys online indiaWebOur results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non-viral based … iron man trailer 4WebApr 12, 2024 · Polymeric gene carriers target the nucleus and express their therapeusis through transcription and translation . Gene carriers are either viral vectors (retro-viruses and adenoviruses) or non-viral vectors. Viral vectors submit their genetic materials to the host cells and are known to attain greater transfection . 18.3.1.2 Polymeric Micelles port orchard hikesWebJun 18, 2024 · Abstract. Functionalized graphene oxide (GO) nanoparticles are being increasingly employed for designing modern drug delivery systems because of their high degree of functionalization, high surface area with … iron man toys for toddlers