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Phospho eif2 alpha antibody

WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ... WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ...

PE anti-EGFR Phospho (Tyr845) Recombinant Antibody

WebPhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. Formulation： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source： Polyclonal, Rabbit,IgG Dilution： IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. WebPhosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important … smart city conneticut https://norcalz.net

Anti-eIF2 alpha (phospho Ser51) antibody (GTX50300) GeneTex

WebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated … WebPhospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb detects endogenous eIF2α only when phosphorylated at Ser51. The antibody does not recognize elF2α phosphorylated at other … WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … hillcrest dance club sun city west

eIF2 alpha Antibody Affinity Biosciences

Category:Anti-eIF2 alpha (phospho Ser48) antibody (GTX50342)

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Phospho eif2 alpha antibody

eIF2 alpha Antibody Affinity Biosciences

WebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2) WebEIF2 alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody; ... All lanes : Anti-EIF2S1 (phospho S51) antibody (ab131505) at 1/500 dilution Lane 1 : K562 cell extract Lane 2 : K562 cell extract treated with IFN alpha

Phospho eif2 alpha antibody

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WebSpecificity. This antibody reacts to human eIF2 alpha/EIF2S1 (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF2 alpha/EIF2S1 without phosphorylation at Ser51. This antibody may also react to mouse or rat Phospho-eIF2 alpha/EIF2S1 (Ser51). Isotype. WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of …

WebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … WebSubunit Structure: Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. …

WebAntibodies are purified by protein A and peptide affinity chromatography. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism of downregulating protein … WebGuarantees. Rabbit polyclonal antibody to eIF2 alpha (phospho Ser51). This antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51. Synthetic …

WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of …

WebPhospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize … hillcrest dallas txWebMay 13, 2024 · For eIF2 phosphorylation, purified PKR and eIF2 (0.08 µg PKR per 10 µg of eIF2) were mixed at room temperature in eIF2 storage buffer supplemented with 0.5 mM ATP, 10 mM MgCl 2 and 5 mM NaF, and ... smart city connect 2021WebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ... hillcrest dallas texasWebBinding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). smart city concept indiaWebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … smart city contractorsWebAnti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications Suitable for: WB, IHC-P, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF or IP Species … hillcrest dc mental healthWebProduct name Anti-elF2 alpha (phospho S52) antibody - N-terminal Description Rabbit polyclonal to elF2 alpha (phospho S52) - N-terminal Host species Rabbit Tested … hillcrest cycle