WebThe cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab137029, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor ® 488 goat anti-rabbit IgG (H&L) ( ab150081 ) at 1/2000 dilution for 30 min at 22°C. WebRab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (7,8). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation, and maturation of phagosome and autophagic vacuoles (8-11).
Rab7 (D95F2) XP® Rabbit mAb Cell Signaling Technology
WebSep 22, 2024 · The switch I/II and interswitch regions of the GTP-loaded Rab7 form the binding interfaces, which correlates with the GTP-dependent interaction of PDZD8 and Rab7. Analysis of the protein ... WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Alexa Fluor® 647 Rabbit monoclonal [EPR7589] to RAB7. Suitable for: ICC/IF. Knockout validated. Reacts with: Human. Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm. 名札入れ 革
High RAS-related protein Rab-7a (RAB7A) expression is a poor …
WebView chapter Purchase book. ... More directly, mutations in the G-protein Rab7 trigger CMT2B, leading to constitutive activity of mutant proteins. Rab7 controls dynein/dynactin … WebJun 26, 2014 · Real-time imaging of control SK-Mel-103 melanoma cells stably expressing GFP-RAB7 (green) and the autophagy protein LC3 labeled in red by fusion to the Cherry protein. Images were captured at 10 min intervals by a DeltaVision RT fluorescence microscope coupled to a CO 2 - and temperature-controlled incubation chamber. WebThe RAB7 cDNA encodes a 207-amino acid protein whose sequence is 99% identical to those of mouse, rat, and dog Rab7 and 61% identical to that of yeast Rab7. Using … 名残惜しい