WebSep 1, 2004 · Polyclonal antibodies against rbcL and rbcS subunit were generated in female BALB/c mice and had no cross-reaction with each other. A total of 81 μg antigens were used and 0.3 ml anti-sera with titer of 1:5000 were yielded. The antibodies were also applicable to study rbcL and rbcS in tobacco plant Nicotiana benthamiana. WebMar 18, 2011 · B) Immunoblot analysis using a specific RbcL antibody. C) Quantification of RbcL signal intensity from three independent subtilisin assays for M. aeruginosa PCC7806 wild type and ΔmcyB mutant. * The starting points were adjusted to 100% D) Control treatment without addition of subtilisin for three hours.

Anti-rbcL Antibody Rabbit anti-Soybean Polyclonal ELISA,WB

WebJul 9, 2008 · Spherical bodies containing RbcL (RCBs; arrowheads) were found in the vacuolar compartment. B and C, Leaf sections were double immunolabeled with anti-GFP and anti-RbcL antibodies. After immunolabeling with anti-GFP antibodies followed by 15-nm gold-conjugated goat anti-rabbit IgG, silver enhancement of gold particles was performed. WebFor coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for … hiller plumbing heating nashville

Effects of CO2 and RuBisCO concentration on cyanobacterial …

WebIP. Immunoprecipitation of Bcl-xL from Jurkat cell extracts, using Bcl-xL Antibody. Lane 1 is the lysate control; lane 2 is antibody alone and lane 3 is antibody plus lysate. Western blot analysis of extracts from Jurkat (human), NIH/3T3 (mouse) or C6 (rat) cells, untreated or etoposide-treated (25 µM, Jurkat) or staurosporine-treated (1 µM ... WebThe rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. The kit contains anti-RbcL antibody, recombinant RbcL protein and PEB buffer for quantitative protein extraction. Application Information. Western blot 1: 5,000-10,000 with standard ECL. WebAug 1, 2024 · The IP and WB assays using a pan-anti-Khib antibody verified that RbcL was indeed Khib-modified (Figure 4 A). The majority of Khib sites occur in the key function regions [46]. For example, the RbcL ε-amino groups, K175, K177, K201, and K252, play an important role in maintaining the stability of the -CO 2-Mg 2+ ternary complex [47]. hiller plumbing knoxville tn phone number