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Choosing Your Fluorescent Proteins for Multi-Color Imaging
WebbSystemic lupus erythematosus (SLE) is an autoimmune disease that affects the cardiovascular, gastrointestinal, hematologic, integumentary, musculoskeletal, neuropsychiatric, pulmonary, renal, and ... WebbScientist with longstanding experience in experimental and computational protein design regarding catalysis, function, binding-affinity and structure. With a focus on the design of … nintendo ds drawn to life
Addgene: Fluorescent Protein Guide
WebbCystic fibrosis (CF) patients develop a severe form of the disease when the cystic fibrosis transmembrane conductance regulator (CFTR) gene is affected by nonsense mutations. Nonsense mutations are responsible for the presence of a premature termination codon (PTC) in the mRNA, creating a lack of functional protein. In this context, translational … Webb"Genetic screens and directed evolution for protein solubility" Curr Opin Chem Biol 7(1), 33-8. 2003. Pédelacq et al. "Engineering soluble proteins for structural genomics," Nature Biotechnology 20, 927-932, September 2002. Waldo et al. "Rapid protein-folding assay using green flourescent protein," Nature Biotechnology 17, 691 - 695, July 1999. Webb12 nov. 2013 · 2) Using warmed PFA. Cold PFA might mess up membrane fluidity, and possibly cause GFP leakage before complete crosslinking of the soluble GFP to the intracellular matrix has occured, not to... nintendo ds emulator for windows 8