Weband co‑workers in 1982,1 is the most widely used fluorescent indicator for intracellular pH. Several characteristics contribute to this widespread utility: The pK a of 7.0 is ideally matched to the normal range of cytoplasmic pH (~6.8–7.4). The fluorescence excitation profile is pH‑dependent (Figure 1), allowing the implemen‑ WebA green organic laboratory experiment was developed inside which students synthesize a sensor for thiols using a microscale, solventless Diels–Alder reaction at room temperature or 37 °C. The molecular probe is easily purified by pillar chromatography ...
A simple optical fiber device for quantitative fluorescence …
Web12 Jun 2015 · SNARF was excited at 543 nm, and fluorescence recorded between 560 and 750 nm. For calibration curves, the ratio of intensities of low-wavelength maximum (at 580 nm) and the high-wavelength maximum (at 630 nm for soluble SNARF, and 650 nm for SNARF-DOPE) was calculated. Web28 Apr 2014 · SNARF-1 is restricted to the cytosolic compartment whereas HPTS allows ratiometric pH measurement of the endosomal/lysosomal compartment. Cell loading with HPTS for 16 hr allows its selective localization in endosomes/lysosomes 4. A typical example of fluorescence recorded from HT-1080 cells loaded with the pH-sensing probes … lock threads
Fluorescence & FISH - Multi Band Filter Sets Chroma Technology …
Web2 Feb 2024 · Data in (A) and (B) normalized to Sodium Green fluorescence under basal conditions (1 mM glucose; ... The ratiometric dye SNARF-5F was excited at 543 nm and emission was collected at 650 nm and 600 nm, which corresponds to the emission maxima of the sensor at pH 9 and pH 6, respectively. Fluo-4 was excited at 488 nm and imaged at … Web3. pH-dependent spectra of water-soluble SNARF Figure S3: pH-dependent fluorescence spectra of water-soluble SNARF. Fluorescence emission spectra of water-soluble SNARF in buffer solutions of different pH upon excitation at 543 nm. All measurements were made in pH-adjusted buffers of the indicated pH values Web3 Apr 2024 · We used fluorescent pH indicators that were observed to have pH-dependent spectral shifts at LAMP temperatures (BCECF-AM and SNARF-1). These dyes have pK a values of pH 6.98 and 7.5, respectively, which are close to neutral pH, and exhibit robust fluorescence changes (>10-fold) through pH 6.0–9.0 (Supplementary Figure S4). lockthis