Thioredoxin fusion expression vector
WebJun 1, 2001 · This unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner. The system is particularly … WebThe pBAD-DEST49 vector features: • The araBAD promoter for tightly regulated expression in E. coli • HP-thioredoxin fusion for improved protein yield and solubility • Enterokinase …
Thioredoxin fusion expression vector
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WebNov 4, 2014 · Overexpression of the his 6-thioredoxin-GSL1 fusion protein in E. coli. The his 6-thioredoxin-GSL1 fusion protein was overexpressed in the E. coli strain BL21 (DE3) using the pET-32a vector. In comparison with thioredoxin alone (Figure 2A, lanes 5-7, right arrow), very little GSL1–thioredoxin fusion protein was expressed (Figure 2A, lanes 2–4), as … WebThe pET-32a (+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are …
WebApr 1, 2000 · This 1.4 kb fragment was cloned into the high‐expression vector pET32a, under control of the T7 promoter. Expression of this plasmid in Escherichia coli BL21(DE3) resulted in the production of a fusion protein (≈62 kDa) consisting of 112 amino acids of thioredoxin and ≈450 amino acids of fragment C. WebFeb 1, 1993 · We have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the ...
WebThis 1.4 kb fragment was cloned into the high-expression vector pET32a, under control of the T7 promoter. Expression of this plasmid in Escherichia coli BL21(DE3) resulted in the production of a fusion protein ( approximately 62 kDa) consisting of 112 amino acids of thioredoxin and approximately 450 amino acids of fragment C. Webthioredoxin gene fusion expression vector, pTRXFUS. The DNA sequence is shown for the 3' -end of the thioredoxin gene, the "linker" region encoding an enterokinase (enteropepti …
WebBackground: To obtain thermal stable, soluble, biologically active hepatitis E virus recombinant antigen using thioredoxin fusion expression system. Methods: HEV ORF2 gene fragment (6964-7126 nt) was inserted into thioredoxin fusion expression vector pThioHisA. The recombinant plasmid was transformed into E. coli BL21 strain. After induction with …
WebJan 18, 2016 · After sub-cloning to Pet102/D.TOPO expression vector, PR coding ORF was expressed in fusion with thioredoxin under T7 promoter control in E. coli. N-terminus thioredoxin fusion will promote protein folding, expression, and solubility as proved by others 26–30. Furthermore, increased solubility will decrease the formation of inclusion … seize the initiative d3WebFeb 10, 2024 · Using a prokaryotic expression vector, pET41a, ThTrx fusion protein containing the glutathione-S-transferase (GST) Tag, was constructed and expressed in E. coli (DE3), with fusion protein expression confirmed via Western blot analysis using antibody specific for GST (Figure 3A–C). After binding of the fusion protein to a nickel column, the ... seize the initiative meaningWebThe popular pET Trx Fusion System 32 is designed for cloning and high-level expression of polypeptide sequences fused with the 109-aa Trx•Tag™ thioredoxin protein. Many proteins that are normally produced in an insoluble form in E. coli tend to become more soluble when fused with the Trx•Tag sequence (LaVallie 1993, Novy 1995). seize the initiative phase